Coding

Part:BBa_K2924031:Design

Designed by: Andreas Nakielski   Group: iGEM19_Duesseldorf   (2019-10-12)


β-lactoglobulin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 535
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 203
    Illegal SapI.rc site found at 178

Design Notes

The sequence is codon-optimized for expression in E. coli

Source

The DNA sequence was acquired by reverse-translating the protein sequence of b-lactoglobulin.

References